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Gel electrophoresis

Our Core Facility has all necessary equipment for 2 dimensional gel electrophoresis. The detection of gels is performed by Bio-Rad ChemiDoc XRS System. We also have Agilent 2100 Bioanalyzer to run protein gel chips, and Agilent 2100 Bioanalyzer for FACS analysis on chips.

The Bio-Rad ChemiDoc XRS System is Bio-Rad’s most advanced imaging system. The system is able to image chemiluminescent, fluorescent and colorimetric samples and comes complete with a compact darkroom, transilluminator and supercooled high resolution 1.44 megapixel CCD camera. The entire system is controlled by the provided Quantity One 1-D analysis software with additional controls on the compact darkroom to control focus, aperture and zoom. Consequently, the ChemiDoc XRS system is extremely flexible and can be used for imaging a diverse range of applications such as Western blotting, DNA/RNA gel electrophoresis, 2-D gel electrophoresis, densitometry, etc. The system is able to detect HRP and AP chemiluminescence, ethidium bromide, SYBR® Green, green fluorescent protein, silver stain and Coomasie brilliant blue staining to name but a few that are frequently used with the above applications.

Bio-Rad ChemiDoc XRS System is equiped with 2D gel analysis software.

Chemidoc Bio-Rad ChemiDoc XRS System