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Sergei Nekhai , Ph.D.

Sergei

Sergei Nekhai, Ph.D. [snekhai@howard.edu] [biosketch]
Director, RCMI Proteomics Core Facility
Associate Professor, Center for Sickle Cell Disease
Howard University, College of Medicine

Sergei Nekhai Molecular Virology Research Group

M.S. Biophysics, State Polytechnic University, St. Petersburg, Russia, 1986
Ph.D. Biophysics, Petersburg Nuclear Physics Institute, Russia, 1994

Office
Rm 6B24
Howard University Hospital
2041 Georgia Ave., NW
Washington, DC 20060

Research Interests

  • Regulation of HIV-1 transcription by host cell CDK2 and protein phosphatase-1
  • Effect of iron chelators and hypoxia on activation of HIV-1 transcription
  • Design of small molecule inhibitors that target phosphatase-1 and inhabit HIV-1
  • Effect of abnormal hypoxic response in Sickle Cell Disease and Chuvash polycythemia in development of pulmonary hypertension
  • Design small molecule PP1 inhibitors to prevent sickling of Sickle Cell Disease red blood cells

Selected Publication

  • Ammosova, T., Yedavalli, V.R.K., Niu, X., Jerebtsova, M., Van Eynde, A., Beullens, M., Bollen, M., Jeang, K.-T., and Nekhai S. 2011. Expression of PP1 inhibitor, cdNIPP1, increases CDK9 Thr186 phosphorylation and inhibits HIV-1 transcription. J. Biol. Chem., in press.
  • Debebe Z, Ammosova T, Breuer D, Lovejoy D, Kalinowski D, Karla P, Kumar K, Jerebtsova M, Ray P, Kashanchi F, Gordeuk V, Richardson DR, Nekhai S. 2011. Iron Chelators of the DpT and BpT Series Inhibit HIV-1 Transcription: Identification of Novel Cellular Targets - Iron, CDK2 and CDK9. Mol Pharmacol., 79:185-96.
  • Jerebtsova M, Klotchenko SA, Artamonova TO, Ammosova T, Washington K, Egorov VV, Shaldzhyan AA, Sergeeva MV, Zatulovskiy EA, Temkina OA, Petukhov MG, Vasin AV, Khodorkovskii MA, Orlov YN, and Nekhai S. 2011. Mass spectrometry and biochemical analysis of RNA polymerase II: targeting by protein phosphatase-1. Mol Cell Biochem., 347:79-87
  • Xu, M., Kashanchi, F., Foster, A., Rotimi, J., Turner, W., Gordeuk, V. R., and Nekhai S. 2010. Hepcidin Induces HIV-1 Transcription Inhibited by Ferroportin. Retrovirology, 7:104.
  • Kasvosve, I., Debebe, Z., Nekhai S., and Gordeuk V.R. 2010. Ferroportin (SLC40A1) Q248H mutation is associated with lower circulating plasma tumor necrosis factor-alpha and macrophage migration inhibitory factor concentrations in African children. Clin Chim. Acta. 411:1248-52
  • Charles, S., Ammosova, T., Cardenas, J., Foster, A., Rotimi, J., Jerebtsova, M., Ayodeji, A. A., Niu, X., Ray, P.E., Gordeuk, V.R., Kashanchi, F., and S. Nekhai 2009. Regulation of HIV-1 transcription at 3% versus 21% oxygen concentration. J. Cell. Physiol , 221:469-479.
  • Debebe, Z., Ammosova., T., Jerebtsova, M., Kurantsin-Mills, J., Niu, X., Charles, S.,Richardson, D. R., Ray, P.E., Goredeuk, V.R., and S. Nekhai. 2007. Iron chelators ICL670 and 311 inhibit HIV-1 transcription. Virology, 36:324-333.
  • Nekhai, S., Bhat, U. G., Ammosova, T., Jerebtsova, M., Niu, X., Radhakrishnan, S. K., Foster, A., Layden, T. J., and A. L. Gartel. 2007. A novel anti-cancer agent ARC antagonizes HIV-1 and HCV. Oncogene, 26:3899-903.
  • Nekhai, S. Jerebtsova, M., Jackson, A., and W. Southerland. 2007. Regulation of HIV-1 Transcription by protein phosphatase-1. Curr HIV Res. 5: 3-9.
  • Ammosova, T., R. Berro, M. Jerebtsova, A. Jackson, S. Charles, Z. Klase, W. Southerland, V. R. Gordeuk, F. Kashanchi, and S. Nekhai. 2006. Phosphorylation of HIV-1 Tat by CDK2 in HIV-1 transcription. Retrovirology 3:78.
  • Ammosova, T., Jerebtsova, M., Beullens, M., Lesage, B., Jackson, A., Kashanchi, F., Southerland, W., Gordeuk, V.R., Bollen, M., and Nekhai, S. 2005. Nuclear targeting of protein phosphatase-1 by HIV-1 Tat protein. J. Biol. Chem. 280:36364-36367.
  • Ammosova, T., Berro, R., Kashanchi, F., and Nekhai, S. 2005. RNA interference directed to CDK2 inhibits HIV-1 transcription. Virology, 341:171-178.
  • Ammosova, T., Washington, K., Debebe, Z., Brady, J., and Nekhai, S. 2005. Dephosphorylation of CDK9 by protein phosphatase 2A and protein phosphatase-1 in Tat-activated HIV-1 transcription. Retrovirology 2: 47.
  • Epie, N., Ammosova, T., Sapir, T., Voloshin, Y., Lane, W.S., Turner, W., Reiner, O., and Nekhai, S. 2005. HIV-1 Tat Interacts With LIS1 Protein. Retrovirology. 2: 6.
  • Ammosova, T., Jerebtsova, M., Beullens, M., Voloshin, Y., Ray, P., Kumar, A., Bollen, M., and  Nekhai, S. 2003. Nuclear protein phosphatase-1 regulates HIV-1 transcription. J. Biol. Chem. 278, 32189-32194.
  • Nekhai, S, Zhou, M., Fernandez, A, Lane, W. S.,  Lamb, N. J. C., Brady, J. and A. Kumar HIV-1 Tat-associated CTD Kinase, CDK2, Phosphorylates CDK7 and Stimulates Tat-mediated Transcription. 2002. Biochem. J., 364, 649-657.